Collection
- Anthony P. "Tony" Pizzo collection, 1824-1994, Date acquired: 10/23/19823
- Children's Home Society of Florida collection, 1862-2019, bulk 1902-19973
- John and Ellen Sheppard Historical Fort Myers Collection, bulk 1865-2000s3
- Northeast Florida Regional Postcard Collection, 1858-20193
- Smith Families of Virginia Letters, 1831-19423
- St. John's Episcopal Church Records, 1832-2002, Date acquired: 10/17/20163
- USF Department of Anthropology African Americans in Florida project, 1864-1998, bulk 1930-19803
- Alex Haley Papers, Circa 1937-19932
- Barnett Family Collection, bulk 1859-19342
- Bryant West Indies Ephemera Collection, 1682-19682
- City of Brandon collection, 1848-20052
- Collection of Spanish Plays, 1835-19412
- Cuban Archives, 1786-1989, bulk 1960-19752
- El Círculo Cubano (Cuban Club) records, 1852-1989, Date acquired: 00/00/2000, bulk 1902-19592
- First United Methodist Church of Fort Myers, bulk 1872-20162
- Florida graphics collection, 1850-19902
- Floridiana Collection, 1730-19702
- John Young Gooch Collection [Photocopy], 1868-19162
- Lopez-Portillo y Lancaster-Jones Collection, 1784-20232
- Marvin and Sybil Weiner Spirit of America Collection -- Puck Magazines, The majority of the material is from the 1880s and 1890s and deals largely with social and political issues of the time., bulk 1878-19162